Biological Database Platform

State Key Laboratory of Silkworm Genome Biology, Southwest University
College of Computer and Information Science, Southwest University
College of Animal Science and Technology, Southwest University
College of Agronomy and Biotechnology, Southwest University

Dr. Tian LI

李田, 博士, 副研究员, 硕士生导师
Tian LI, Ph.D.
Associate Professor
State Key Laboratory of Silkworm Genome Biology, Southwest University
Chongqing Key Laboratory of Microsporidia Infection and Control, Southwest University
Institute of Sericulture and Systems Biology, Southwest University
Email: lit{at}
Academic field: Microbiology, Biochemistry and Molecular Biology, Genomics, Bioinformatics
  • 2014-2015 Visting Scholar TROEMEL Lab, Division of Biological Science, University of California, San Diego.
  • 2014 Associate Professor State Key Laboratory of Silkworm Genome Biology, and Institute of Sericulture and Systems Biology, Southwest University, China.
  • 2009 Lecturer State Key Laboratory of Silkworm Genome Biology, Institute of Sericulture and Systems Biology, Southwest University, China.
  • 2009 Ph.D. Biochemistry and Molecular Biology (Supervisor: Prof. Zeyang Zhou), Institute of Sericulture and Systems Biology, Southwest University, China.
  • 2005 M.S. Microbiology (Supervisor: Prof. Zeyang Zhou), Institute of Sericulture and Systems Biology, Southwest University, China.
  • 2003 B.Sc. Animal Medicine, College of Animal Science and Technology, Southwest Agricultral University, China.
  • 2003 B.Sc. Computer Science and Technology, College of Computer and Information, Southwest Agricultral University, China.

Microsporidia are a group of obligated intracellular parasites that can infected nearly all animals, including silkworm, bee and human. Our research focuses mainly on microsporidia of economic importance (Nosema bombycis and Vairimorpha necatrix, pathogens of silkworm) and veterinary (Encephalitozoon sp., a common vertebrate pathogen) to understand their genomics, pathogensis and host response, and diagnosis and prevention.

  • Microsporidian Infection and Prevention
    • Secreted proteins are important virulent factors. How do microsporidia regulate host immune, metabolism and development with secreted proteins? And how does host respond to the interference?
  • Microsporidian Transovarial Transmission
    • Microsporidia can be vertically transmitted via invading ovary and oocyte of economy-important insects, including silkworm and honeybee, and cause descendant microsporidiosis, which is a great threat to industry. However, molecular mechanism of the transovarial transmission remains unclear. We are making efforts to dissect the transovarial process, identify factors that mediate the invasion, and construct resistant breeds by blocking the transmission process.
  • Genomics and Bioinformatics
    • Sequence and analyze microsporidian genome, transcriptome and proteome to understand the molecular and genetic base of pathgenesis and host response.
    • Develop bioinfomatic methods and tools, and build biological databases: BioDB Platform (SilkPathDB, MorusDB, MnTEdb, PamulDB, qPrimerDB, ...)
  • Natural Scientific Fund, China, 2018 – 2021.
  • Natural Scientific Fund, China, 2015 - 2018.
  • Fundamental Research Funds for the Central Universities, 2015 - 2017.
  • Natural Scientific Fund, China, 2010 - 2013.
  • Natural Scientific Fund, China, 2010 - 2013.
  • Chongqing Natural Science Foundation, 2010 - 2013.
  • Fundamental Research Funds for the Central Universities, 2010 - 2012.
  • The Research Fund for the Doctoral Program, 2009.
  1. Haoyu Chao, Tian Li, Chaoyu Luo, Hualei Huang, Yingfei Ruan, Xiaodong Li, Yue Niu, Yonghai Fan, Wei Sun, Kai Zhang, Jiana Li, Cunmin Qu and Kun Lu*. BrassicaEDB: A Gene Expression Database for Brassica Crops. Int. J. Mol. Sci. 2020, 21(16), 5831.
  2. Yinze Han, Hailong Gao, Jinzhi Xu, Jian Luo, Bing Han, Jialing Bao, Guoqing Pan, Tian Li*, Zeyang Zhou*. Innate and adaptive immune responses against microsporidia infection in mammals. Front. Microbiol.. 2020 June 26.
  3. Qiang He, Jian Luo, Jin-Zhi Xu, Xian-Zhi Meng, Guo-Qing Pan, Tian Li*, Ze-Yang Zhou*. In-vitro cultivation of Nosema bombycis sporoplasms: a method for potential genetic engineering of microsporidia. J Invertebr Pathol. 2020 Jun 6;174:107420. doi: 10.1016/j.jip.2020.107420. [PubMed]
  4. Qiang He, Jian Luo, Jin-Zhi Xu, Xian-zhi Meng, Guo-Qing Pan, Tian Li*, Ze-Yang Zhou*. Characterization of Hsp70 gene family provides insight into its functions related to microsporidian proliferation. J Invertebr Pathol. 2020, 107394. doi: 10.1016/j.jip.2020.107394. [PubMed]
  5. Qiang He, Jian Luo, Jin-Zhi Xu, Chun-xia Wang, Xian-zhi Meng, Guo-Qing Pan, Tian Li*, Ze-Yang Zhou*. Morphology and Transcriptome Analysis of Nosema bombycis Sporoplasm and Insights into the Initial Infection of Microsporidia. mSphere. 2020 Feb 12;5(1). pii: e00958-19. doi: 10.1128/mSphere.00958-19. [PubMed] [mSphere]
  6. Yue Song, Yunlin Tang, Qiong Yang, Tangxin Li, Zhangshuai He, Yujiao Wu, Qiang He, Tian Li, Chunfeng Li, Mengxian Long, Jie Chen, Junhong Wei, Jialing Bao, Zigang Shen, Xianzhi Meng, Guoqing Pan*, Zeyang Zhou*. Proliferation Characteristics of the Intracellular Microsporidian Pathogen Nosema Bombycis in Congenitally Infected Embryos. J Invertebr Pathol. 2020, 169:107310. doi: 10.1016/j.jip.2019.107310. [PubMed]
  7. Zhihong Li, Youpeng Fan, Junhong Wei, Xionge Mei, Qiang He, Yonghua Zhang, Tian Li, Mengxian Long, Jie Chen, Jialing Bao, Guoqing Pan, Chunfeng Li* and Zeyang Zhou*. Baculovirus Utilizes Cholesterol Transporter NIEMANN–Pick C1 for Host Cell Entry. Front Microbiol. 2019 Dec 5;10:2825. doi: 10.3389/fmicb.2019.02825. eCollection 2019. [PubMed]
  8. Wenjia Ni, Jialing Bao, Biying Mo, Lulu Liu, Tian Li, Guoqing Pan, Jie Chen, ZeyangZhou*. Hemocytin facilitates host immune responses against Nosema bombycis. Dev Comp Immunol. 2020, 103:103495. doi: 10.1016/j.dci.2019.103495. [PubMed]
  9. Zhangshuai He, Qi Ni, Yue Song, Rui Wang, Yunlin Tang, Yujiao Wu, Lulu Liu, Jialing Bao, Jie Chen, Mengxian Long, Junhong Wei, Chunfeng Li, Tian Li, Zeyang Zhou, GuoqingPan. Development of a nucleic acid lateral flow strip for rapid, visual detection of Nosema bombycis in silkworm eggs. J Invertebr Pathol. 2019, 164:59-65. doi: 10.1016/j.jip.2019.04.004. [PubMed]
  10. Tian Li*, Xiao-Fei Xu, Hui-Hui Du, Li Li, Neng-Zhang Li, Ze-Yang Zhou and Yuan-Yi Peng*. PamulDB: a comprehensive genomic resource for the study of human- and animal-pathogenic Pasteurella multocida. Database, 2019, baz025. doi: 10.1093/database/baz025. [PubMed]
  11. Qiang He, Charles R. Vossbrinck, Qiong Yang, Xian-Zhi Meng, Jian Luo, Guo-Qing Pan, Ze-Yang Zhou*, Tian Li*. Evolutionary and functional studies on microsporidian ATP-binding cassettes: Insights into the adaptation of microsporidia to obligated intracellular parasitism. Infect Genet Evol, 2019, 68: 136-144. doi: 10.1016/j.meegid.2018.12.022. [PubMed]
  12. Huang Y, Zheng S, Mei X, Yu B, Sun B, Li B, Wei J, Chen J, Li T, Pan G, Zhou Z, Li C. A secretory hexokinase plays an active role in the proliferation of Nosema bombycis. PeerJ, 2018, 6:e5658. doi: 10.7717/peerj.5658. eCollection 2018. [PubMed]
  13. Xu WF, Ren HS, Ou T, Lei T, Wei JH, Huang CS, Li T, Strobel G, Zhou ZY, Xie J. Genomic and Functional Characterization of the Endophytic Bacillus subtilis 7PJ-16 Strain, a Potential Biocontrol Agent of Mulberry Fruit Sclerotiniose. Microb Ecol. 2018, 3. doi: 10.1007/s00248-018-1247-4. [PubMed]
  14. Xian-Zhi Meng, Bo Luo, Xiang-You Tanga, Qiang He, Ting-Rong Xiong, Zhuo-Ya Fang, Guoqing Pan, Tian Li*, Ze-Yang Zhou*. Pathological analysis of silkworm infected by two microsporidia Nosema bombycis CQ1 and Vairimorpha necatrix BM. J Invertebr Pathol. 2018, 153: 75-84. doi: 10.1016/j.jip.2017.12.005. [PubMed]
  15. Kun Lu, Tian Li, Jian He, Wei Chang, Rui Zhang, Miao Liu, Mengna Yu, Yonghai Fan, Jinqi Ma, Wei Sun, Cunmin Qu, Liezhao Liu, Nannan Li, Ying Liang, Rui Wang, Wei Qian, Zhanglin Tang, Xinfu Xu, Bo Lei, Kai Zhang, Jiana Li. qPrimerDB: a thermodynamics-based gene-specific qPCR primer database for 147 organisms. Nucleic Acids Research, 2017, gkx725. [PubMed] [PDF]. (Authors contributed equally to this paper.)
  16. Tian Li, Guo-Qing Pan, Charles R. Vossbrinck, Jin-Shan Xu, Chun-Feng Li, Jie Chen, Meng-Xian Long, Ming Yang, Xiao-Fei Xu, Chen Xu, Bettina A. Debrunner-Vossbrinck, Ze-Yang Zhou. SilkPathDB: a comprehensive resource for the study of silkworm pathogens. Database. 2017(1): bax001. [PubMed] [PDF]
  17. Ying Wang, Huixia Geng, Xiaoqun Dang, Heng Xiang, Tian Li, Guoqing Pan, Zeyang Zhou. Comparative Analysis of the Proteins with Tandem Repeats from 8 Microsporidia and Characterization of a Novel Endospore Wall Protein Colocalizing with Polar Tube from Nosema bombycis. J Eukaryot Microbiol. 2017, 64(5):707-715. [PubMed] [PDF]
  18. Donglin Yang, Lixia Pan, Pai Peng, Xiaoqun Dang, Chunfeng Li, Tian Li, Mengxian Long, Jie Chen, Yujiao Wu, Huihui Du, Bo Luo, Yue Song, Rui Tian, Jie Luo, Zeyang Zhou and Guoqing Pan. Interaction between SWP9 and Polar Tube Proteins of the Microsporidian Nosema bombycis and Function of SWP9 as a Scaffolding Protein Contribute to Polar Tube Tethering to the Spore Wall. Infect Immun. 2017, 85(3), pii: e00872-16. [PubMed] [PDF]
  19. ZhiHong Li, GuoQing Pan, ZhenGang Ma, Bing Han, Bin Sun, Qi Ni, Jie Chen, Tian Li, TongBao Liu, MengXian Long, ChunFeng Li, ZeYang Zhou. Comparative proteomic analysis of differentially expressed proteins in the Bombyx mori fat body during the microsporidia Nosema bombycis infection. J Invertebr Pathol. 2017, 149:36-43. doi:10.1016/j.jip.2017.06.009. [PubMed]
  20. Bing Han, Kang Zhou, Zhihong Li, Bin Sun, Qi Ni, Xianzhi Meng, Guoqing Pan, Chunfeng Li, Mengxian Long, Tian Li, Congzhao Zhou, Weifang Li, Zeyang Zhou. Characterization of the First Fungal Glycosyl Hydrolase Family 19 Chitinase (NbchiA) from Nosema bombycis (Nb). J Eukaryot Microbiol. 2016, 63(1):37-45. doi: 10.1111/jeu.12246. [PubMed]
  21. Huihui Du, Rendong Fang, Tingting Pan, Tian Li, Nengzhang Li, Qiang He, Rui Wu, Yuanyi Peng, and Zeyang Zhou. Comparative genomics analysis of two different virulent bovine Pasteurella multocida isolates. International Journal of Genomics. 2016(2016), accepted. [Link]
  22. Xu J, He Q, Ma Z, Li T, Zhang X, Debrunner-Vossbrinck BA, Zhou Z, Vossbrinck CR. The Genome of Nosema sp. Isolate YNPr: A Comparative Analysis of Genome Evolution within the Nosema/Vairimorpha Clade. PLoS One. 2016, 11(9):e0162336. doi: 10.1371/journal.pone.0162336. [PubMed]
  23. Qiwei Zeng, Hongyu Chen, Chao Zhang, Minjing Han, Tian Li, Xiwu Qi, Zhonghuai Xiang, Ningjia He. Definition of Eight Mulberry Species in the Genus Morus by Internal Transcribed Spacer-Based Phylogeny. PLoS One. 2015, 10(8):e0135411. doi: 10.1371/journal.pone.0135411. eCollection 2015. [PubMed]
  24. Ma B, Li T, Xiang Z, He N. MnTEdb, a collective resource for mulberry transposable elements. DATABASE. 2015, pii: bav004. doi: 10.1093/database/bav004. [PubMed]
  25. Liu T, Xu J, Luo B, Chen J, Li Z, Li T, Pan G, Li X, Zhou Z. Nosema sp. PM-1, a new isolate of microsporidian from infected Papilio machaon Linnaeus, based on ultrastructure and molecular identification. Acta Parasitol. 2015, 60(2): 330-336.
  26. Wang Y, Dang X, Luo B, Li C, Long M, Li T, Li Z, Pan G, Zhou Z. Characterization of a novel otubain-like protease with deubiquitination activity from Nosema bombycis (Microsporidia). Parasitol Res. 2015. [Epub ahead of print].
  27. 周泽扬,潘国庆†,李田†,许金山†.《家蚕微孢子虫基因组生物学》. 科学出版社. 2014, ISBN 978-7-03-042451-8. (†副主编).
  28. Wang Y, Dang X, Ma Q, Liu F, Pan G, Li T, Zhou Z.Characterization of a novel spore wall protein NbSWP16 with proline-rich tandem repeats from Nosema bombycis (microsporidia). Parasitology. 2014, 3:1-9. [PubMed]
  29. Tian Li, Xiwu Qi, Qiwei Zeng, Zhonghuai Xiang, Ningjia He. MorusDB: A resource for mulberry genomics and genome biology. DATABASE. 2014, bau054, doi: 10.1093/database/bau054. [PubMed]
  30. Bo Luo, Handeng Liu, Guoqing Pan, Tian Li, Zeng Li, Xiaoqun Dang, Tie Liu, Zeyang Zhou. Morphological and molecular studies of Vairimorpha necatrix BM, a new strain of the microsporidium V. necatrix (microsporidia, burenellidae) recorded in the silkworm, Bombyx mori. Exp Parasitol. 2014, pii: S0014-4894(14)00115-5. doi: 10.1016/j.exppara.2014.05.001. [PubMed]
  31. Xiang H, Zhang R, De Koeyer D, Pan G, Li T, Liu T, Zhou Z. New evidence on the relationship between Microsporidia and Fungi: a genome-wide analysis by DarkHorse software. Can J Microbiol. 2014, 14:1-12. [PubMed]
  32. Zhengang Ma, Chunfeng Li, Guoqing Pan, Zhihong Li, Bing Han, Jinshan Xu, Xiqian Lan, Jie Chen, Donglin Yang, Quanmei Chen, Qi Sang, Xiaocun Ji, Tian Li, Mengxian Long, Zeyang Zhou. 2013. Genome-wide transcriptional response of silkworm (Bombyx mori) to infection by the microsporidian Nosema bombycis. PloS ONE. 2013, 8(12): e84137. [PubMed]
  33. Chen J, Geng L, Long M, Li T, Li Z, Yang D, Ma C, Wu H, Ma Z, Li C, Pan G, Zhou Z. Identification of a novel chitin-binding spore wall protein (NbSWP12) with a BAR-2 domain from Nosema bombycis (microsporidia). Parasitology. 2013, 140(11): 1394-402.
  34. Yang D, Dang X, Tian R, Long M, Li C, Li T, Chen J, Li Z, Pan G, Zhou Z. Development of an approach to analyze the interaction between Nosema bombycis (microsporidia) deproteinated chitin spore coats and spore wall proteins. J Invertebr Pathol. 2013, pii:S0022-2011(13)00149-3.
  35. LI Zhi-Hong, MA Zhen-Gang, LI Wan-Jun, LIU Jun, HAN Bing, LI Tian, PAN Guo-Qing, LI Chun-Feng, ZHOU Ze-Yang. Analysis and Identification of the Differentially Expressed Hemolymph Proteins of Silkworm (Bombyx mori) Infected by Nosema bombycis. Science of Sericulture. 2013, 39(5): 95-101.
  36. GENG Li-Na, WU Hai-Jing, LIU Ting, TIAN Rui, LI Tian, PAN Guo-Qing, ZHOU Ze-Yang. Two-dimensional Electrophoresis Analysis of Nosema bombycis Spore Coat Proteins Prepared by Germination Method and Glas Beads Breaking Method. Science of Sericulture. 2013, 39(5): 0928-0934.
  37. LUO Jie, DENG Yuan-Hong, HUANG Wei, LI Tian, YANG Qiong, YIN Mei, PAN Guo-Qing, LI Chun-Feng, ZHOU Ze-Yang. Sequence Analysis and Subcellular Localization of a Novel Nosema bombycis Gene in Saccharomyces cerevisiae Cells. Science of Sericulture. 2013, 39(2): 0302-0309.
  38. He N, Zhang C, Qi X, Zhao S, Tao Y, Yang G, Lee TH, Wang X, Cai Q, Li D, Lu M, Liao S, Luo G, He R, Tan X, Xu Y, Li T, Zhao A, Jia L, Fu Q, Zeng Q, Gao C, Ma B, Liang J, Wang X, Shang J, Song P, Wu H, Fan L, Wang Q, Shuai Q, Zhu J, Wei C, Zhu-Salzman K, Jin D, Wang J, Liu T, Yu M, Tang C, Wang Z, Dai F, Chen J, Liu Y, Zhao S, Lin T, Zhang S, Wang J, Wang J, Yang H, Yang G, Wang J, Paterson AH, Xia Q, Ji D, Xiang Z. Draft genome sequence of the mulberry tree Morus notabilis. Nat Commun. 2013, 4:2445. doi: 10.1038/ncomms3445.
  39. LI Tian, QI Xiao-Ran, TAO Mei-Lin, LIU Xian-Lin, KANG Ding-Rong, QIN Guo-Wei, PAN Guo-Qing, ZHOU Ze-Yang. A Comparative Genomics Analysis of Secreted Proteins from Four Microsporidia. Science of Sericulture. 2013, 39(3): 0527-0536.
  40. Guoqing Pan*, Jinshan Xu*, Tian Li*, Qingyou Xia, Shao-Lun Liu, Guojie Zhang, Songgang Li, Chunfeng Li, Handeng Liu, Liu Yang, Tie Liu, Xi Zhang, Zhengli Wu, Wei Fan, Xiaoqun Dang, Heng Xiang, Meilin Tao, Yanhong Li, Junhua Hu, Zhi Li, Lipeng Lin, Jie Luo, Lina Geng, LinLing Wang, Mengxia Long, Yongji Wan, Ningjia He, Ze Zhang, Cheng Lu, Patrick J Keeling, Jun Wang, Zhonghuai Xiang and Zeyang Zhou. Comparative genomics of parasitic silkworm microsporidia reveal an association between genome expansion and host adaptation. BMC Genomics. 2013, 14: 186. (*Co-first authors).
  41. Dang X, Pan G, Li T, Lin L, Ma Q, Geng L, He Y, Zhou Z. Characterization of a subtilisin-like protease with apical localization from microsporidian Nosema bombycis. J Invertebr Pathol. 2013, 112(2): 166-174.
  42. LI Tian, LIU Xian-Lin, HAN Bing, QI Xiao-Ran, DANG Xiao-Qun, KANG Ding-Rong, PAN Guo-Qing, ZHOU Ze-Yang. A Genome-wide Prediction and Analysis on Secreted Proteins of Nosema bombycis. Science of Sericulture. 2013, 39(2): 0295-0301.
  43. MA Qiang, LI Tian, DANG Xiao-Qun, PAN Guo-Qing, XU Jing-Shan, ZHOU Ze-Yang. A Method Suitable for Extracting Membrane Protein from Nosema bombycis. Science of Sericulture. 2013, 39(2): 0319 - 0323.
  44. GENG Li-Na, WU Hai-Jing, MA Cheng, LIU Ting, DANG Xiao-Qun, QI Xiao-Ran, LI Tian, PAN Guo-Qing, ZHOU Ze-Yang. Comparative Analysis of Gene Sequences of Related Enzymes in O-mannosylation Pathway of Microsporidian. Science of Sericulture. 2012, 38(03): 158-168.
  45. Lin L, Pan G, Li T, Dang X, Deng Y, Ma C, Chen J, Luo J, Zhou Z. The protein import pore Tom40 in the Microsporidian Nosema bombycis. J Eukaryot Microbiol. 2012, 59(3):251-257.
  46. Handeng Liu, Guoqing Pan, Tian Li, Wei Huang, Bo Luo, Zeyang Zhou. Ultrastructure, chromosomal karyotype, and molecular phylogeny of a new isolate of microsporidian Vairimorpha sp. BM (Microsporidia, Nosematidae) from Bombyx mori in China. Parasitol Res. 2012, 110(1):205-210.
  47. Zhi Li, Guoqing Pan, Tian Li, Wei Huang, Jie Chen, Lina Geng, Donglin Yang, Linling Wang, Zeyang Zhou. SWP5, A Novel Spore Wall Protein Interaction with Polar Tube in the Parasite Nosema bombycis (Microsporidia). Eukaryotic Cell. 2011, 11: 229-237.
  48. Xiang H, Pan G, Zhang R, Xu J, Li T, Li W, Zhou Z, Xiang Z. Natural selection maintains the transcribed LTR retrotransposons in Nosema bombycis. J Genet Genomics. 2010, 37: 305-314.
  49. Tian Li, Xiaoqun Dang, Jinshan Xu, Handeng Liu, Guoqing Pan, Zeyang Zhou. Mitochondrial Pyruvate Dehydrogenase E1 of Nosema bombycis - a Marker in Microsporidian Evolution. Current Zoology. 2009, 55: 423-429.
  50. LI Tian, PAN Guo-Qing, DANG Xiao-Qun, ZHOU Ze-Yang. cDNA Library Construction and EST Analysis of Bombyx mori Silk Gland Heavily Infected byNosema bombycis. Science of Sericulture. 2009, 35(2): 320-327.
  51. Xia Q, Zhou Z, Lu C, Cheng D, Dai F, Li B, Zhao P, Zha X, Cheng T, Chai C, Pan G, Xu J, Liu C, Lin Y, Qian J, Hou Y, Wu Z, Li G, Pan M, Li C, Shen Y, Lan X, Yuan L, Li T, Xu H, Yang G, et al. A draft sequence for the genome of the domesticated silkworm (Bombyx mori). Science. 2004, 306: 1937-1940.
Last update: Jun 06, 2020.