qPrimerDB - qPCR Primer Database

A resource for real-time quantitative PCR primers

Version: 1.1

Birds_and_Reptiles

Anas platyrhynchos
Gallus gallus
Meleagris gallopavo
Pelodiscus sinensis
Taeniopygia guttata

Fish

Astyanax mexicanus
Danio rerio
Gadus morhua
Gasterosteus aculeatus
Latimeria chalumnae
Lepisosteus oculatus
Oreochromis niloticus
Oryzias latipes
Petromyzon marinus
Poecilia formosa
Takifugu rubripes
Xiphophorus maculatus

Insecta

Acromyrmex echinatior
Aedes aegypti
Anopheles coluzzii
Anopheles stephensi
Apis dorsata
Apis florea
Apis mellifera
Athalia rosae
Bactrocera cucurbitae
Bactrocera dorsalis
Bombus terrestris
Bombyx mori
Camponotus floridanus
Cerapachys biroi
Ceratitis capitata
Chilo suppressalis
Danaus plexippus
Diaphorina citri
Drosophila ananassae
Drosophila erecta
Drosophila grimshawi
Drosophila melanogaster
Drosophila mojavensis
Drosophila sechellia
Drosophila simulans
Drosophila virilis
Drosophila willistoni
Drosophila yakuba
Fopius arisanus
Harpegnathos saltator
Heliconius melpomene
Linepithema humile
Macrocentrus cingulum
Megachile rotundata
Megaselia scalaris
Melitaea cinxia
Microplitis demolitor
Musca domestica
Nasonia vitripennis
Pediculus humanus
Plutella xylostella
Pogonomyrmex barbatus
Rhodnius prolixus
Solenopsis invicta
Tribolium castaneum
Vollenhovia emeryi
Wasmannia auropunctata
Zootermopsis nevadensis

Mammalia

Ailuropoda melanoleuca
Bos taurus
Callithrix jacchus
Canis familiaris
Cavia porcellus
Chlorocebus sabaeus
Choloepus hoffmanni
Dasypus novemcinctus
Dipodomys ordii
Echinops telfairi
Equus caballus
Erinaceus europaeus
Felis catus
Gorilla gorilla
Homo sapiens
Ictidomys tridecemlineatus
Loxodonta africana
Macaca mulatta
Microcebus murinus
Monodelphis domestica
Mus musculus
Myotis lucifugus
Nomascus leucogenys
Ornithorhynchus anatinus
Oryctolagus cuniculus
Otolemur garnettii
Ovis aries
Pan troglodytes
Papio anubis
Pongo abelii
Procavia capensis
Pteropus vampyrus
Rattus norvegicus
Sarcophilus harrisii
Sus scrofa
Tarsius syrichta
Tupaia belangeri
Tursiops truncatus
Vicugna pacos

Other_animals

Anolis carolinensis
Caenorhabditis briggsae
Caenorhabditis elegans
Ciona intestinalis
Ciona savignyi
Ixodes scapularis
Lottia gigantea
Mnemiopsis leidyi
Octopus bimaculoides
Sarcoptes scabiei
Strongylocentrotus purpuratus
Strongyloides ratti
Tetranychus urticae
Xenopus tropicalis

Fungi

Saccharomyces cerevisiae

Eudicotyledons

Aethionema arabicum
Amaranthus hypochondriacus
Arabidopsis halleri
Arabidopsis lyrata
Arabidopsis thaliana
Arachis duranensis
Arachis ipaensis
Beta vulgaris
Boechera stricta
Brassica napus
Brassica oleracea capitata
Brassica oleracea italica
Brassica rapa
Camellia sinensis
Capsella grandiflora
Capsella rubella
Carica papaya
Chenopodium quinoa
Cicer arietinum
Citrus clementina
Citrus sinensis
Coffea canephora
Cucumis melo
Cucumis sativus
Cucurbita moschata
Daucus carota
Eucalyptus grandis
Eutrema salsugineum
Fragaria vesca
Glycine max
Gossypium hirsutum
Gossypium raimondii
Kalanchoe fedtschenkoi
Kalanchoe laxiflora
Linum usitatissimum
Lotus japonicus
Malus domestica
Manihot esculenta
Medicago truncatula
Mimulus guttatus
Nicotiana tabacum
Phaseolus vulgaris
Populus deltoides
Populus trichocarpa
Prunus persica
Ricinus communis
Salix purpurea
Sida fallax
Solanum lycopersicum
Solanum tuberosum
Thellungiella parvula
Theobroma cacao
Trifolium pratense
Vigna angularis
Vigna radiata
Vitis vinifera

Monocotyledons

Aegilops tauschii
Ananas comosus
Brachypodium distachyon
Brachypodium stacei
Hordeum vulgare
Leersia perrieri
Musa acuminata
Oropetium thomaeum
Oryza barthii
Oryza brachyantha
Oryza glaberrima
Oryza glumaepatula
Oryza indica
Oryza longistaminata
Oryza meridionalis
Oryza punctata
Oryza rufipogon
Oryza sativa
Panicum hallii
Setaria italica
Setaria viridis
Sorghum bicolor
Spirodela polyrhiza
Triticum aestivum
Triticum urartu
Zea mays
Zostera marina

Other_plants

Amborella trichopoda
Chlamydomonas reinhardtii
Cyanidioschyzon merolae
Marchantia polymorpha
Micromonas
Micromonas pusilla
Ostreococcus lucimarinus
Physcomitrella patens
Selaginella moellendorffii
Volvox carteri

qPrimerDB BLAST

Version: ncbi-blast-2.7.1+

Birds_and_Reptiles

Anas platyrhynchos
Gallus gallus
Meleagris gallopavo
Pelodiscus sinensis
Taeniopygia guttata

Fish

Astyanax mexicanus
Danio rerio
Gadus morhua
Gasterosteus aculeatus
Latimeria chalumnae
Lepisosteus oculatus
Oreochromis niloticus
Oryzias latipes
Petromyzon marinus
Poecilia formosa
Takifugu rubripes
Xiphophorus maculatus

Insecta

Acromyrmex echinatior
Aedes aegypti
Anopheles coluzzii
Anopheles stephensi
Apis dorsata
Apis florea
Apis mellifera
Athalia rosae
Bactrocera cucurbitae
Bactrocera dorsalis
Bombus terrestris
Bombyx mori
Camponotus floridanus
Cerapachys biroi
Ceratitis capitata
Chilo suppressalis
Danaus plexippus
Diaphorina citri
Drosophila ananassae
Drosophila erecta
Drosophila grimshawi
Drosophila melanogaster
Drosophila mojavensis
Drosophila sechellia
Drosophila simulans
Drosophila virilis
Drosophila willistoni
Drosophila yakuba
Fopius arisanus
Harpegnathos saltator
Heliconius melpomene
Linepithema humile
Macrocentrus cingulum
Megachile rotundata
Megaselia scalaris
Melitaea cinxia
Microplitis demolitor
Musca domestica
Nasonia vitripennis
Pediculus humanus
Plutella xylostella
Pogonomyrmex barbatus
Rhodnius prolixus
Solenopsis invicta
Tribolium castaneum
Vollenhovia emeryi
Wasmannia auropunctata
Zootermopsis nevadensis

Mammalia

Ailuropoda melanoleuca
Bos taurus
Callithrix jacchus
Cavia porcellus
Chlorocebus sabaeus
Choloepus hoffmanni
Dasypus novemcinctus
Dipodomys ordii
Echinops telfairi
Equus caballus
Erinaceus europaeus
Felis catus
Gorilla gorilla
Homo sapiens
Ictidomys tridecemlineatus
Loxodonta africana
Macaca mulatta
Microcebus murinus
Monodelphis domestica
Mus musculus
Myotis lucifugus
Nomascus leucogenys
Ornithorhynchus anatinus
Oryctolagus cuniculus
Otolemur garnettii
Ovis aries
Pan troglodytes
Papio anubis
Pongo abelii
Procavia capensis
Pteropus vampyrus
Rattus norvegicus
Sarcophilus harrisii
Sus scrofa
Tupaia belangeri
Tursiops truncatus
Vicugna pacos

Other_animals

Anolis carolinensis
Caenorhabditis briggsae
Caenorhabditis elegans
Ciona intestinalis
Ciona savignyi
Ixodes scapularis
Lottia gigantea
Mnemiopsis leidyi
Octopus bimaculoides
Sarcoptes scabiei
Strongylocentrotus purpuratus
Strongyloides ratti
Tetranychus urticae
Xenopus tropicalis

Fungi

Saccharomyces cerevisiae

Eudicotyledons

Aethionema arabicum
Amaranthus hypochondriacus
Arabidopsis halleri
Arabidopsis lyrata
Arabidopsis thaliana
Arachis duranensis
Arachis ipaensis
Boechera stricta
Brassica napus
Brassica oleracea capitata
Brassica oleracea italica
Brassica rapa
Camellia sinensis
Capsella grandiflora
Capsella rubella
Carica papaya
Chenopodium quinoa
Cicer arietinum
Citrus clementina
Citrus sinensis
Coffea canephora
Cucumis melo
Cucumis sativus
Cucurbita moschata
Daucus carota
Eucalyptus grandis
Eutrema salsugineum
Fragaria vesca
Glycine max
Gossypium hirsutum
Gossypium raimondii
Kalanchoe fedtschenkoi
Kalanchoe laxiflora
Linum usitatissimum
Lotus japonicus
Malus domestica
Manihot esculenta
Medicago truncatula
Mimulus guttatus
Nicotiana tabacum
Phaseolus vulgaris
Populus deltoides
Populus trichocarpa
Prunus persica
Ricinus communis
Salix purpurea
Sida fallax
Solanum lycopersicum
Solanum tuberosum
Thellungiella parvula
Theobroma cacao
Trifolium pratense
Vigna angularis
Vigna radiata
Vitis vinifera

Monocotyledons

Aegilops tauschii
Ananas comosus
Brachypodium distachyon
Brachypodium stacei
Hordeum vulgare
Leersia perrieri
Musa acuminata
Oropetium thomaeum
Oryza barthii
Oryza brachyantha
Oryza glaberrima
Oryza glumaepatula
Oryza longistaminata
Oryza meridionalis
Oryza punctata
Oryza rufipogon
Panicum hallii
Setaria italica
Setaria viridis
Sorghum bicolor
Spirodela polyrhiza
Triticum aestivum
Triticum urartu
Zea mays
Zostera marina

Other_plants

Amborella trichopoda
Chlamydomonas reinhardtii
Cyanidioschyzon merolae
Marchantia polymorpha
Ostreococcus lucimarinus
Physcomitrella patens
Selaginella moellendorffii
Volvox carteri
   Program:     Expect:     Word size:  [Integer;   blastn: >=4;   others: >=2]

Output Format:  Standard      Table   [ Best Hit (>=1): Best Match (>=1): ]

2. Enter sequence below in FASTA format (EXAMPLE SEQUENCE), or enter qPDB identifiers each with a prefix "ID:" (EXAMPLE IDs).

or load local sequence file in FASTA format:

3.       

qPrimerDB BLAST is a set of tools built with NCBI-BLAST+, and used for aligning and searching homologous sequences in the database. It supports aligning aginst multiple databases.

The NCBI-BLAST+ is buit with the latest version of NCBI BLAST+, ncbi-blast-2.7.1+-x64-linux. It supports searching against multiple databases in one run. The NCBI BLAST+ is an optimized version of the former NCBI BLAST, which is not supported by the National Center for Biotechnology Information (NCBI).